It came after a U.S. air strike in Sangin killed at least 18 civilians, mostly women and children, in February. All the victims, which included women and children, were civilians and they were from one family.
But Bob Purtiman, a spokesman for the American military in Afghanistan, dismissed the official assertions.
On July 24, Afghan officials said, nine civilians were killed in a USA airstrike on a prayer ceremony held in Haska Meena, near the border with Pakistan, by relatives of ISIS members who had been killed. Officials added that because the vehicles were in the middle of nowhere "there was zero chance of civilian casualties".
He said it was the "second false claim" of civilian deaths in the district within the past three weeks.
He reiterated that US forces take all allegations of civilian casualties seriously and review each case fully, as well as take precautions in a way to minimize harm to Afghan civilians.
The New York Times reported earlier Friday that Afghan officials said US aircraft killed 16 civilians as they tried to flee the area.
The Resolute Support and United States forces in Afghanistan have not commented so far. Back in February، American airstrikes also killed 22 civilians in the same province.
The Taliban, seeking to restore Islamic rule after their 2001 ouster, rejected statements that the attack on Mirza Olang was a joint operation with the local affiliate of Islamic State, saying they acted alone and had targeted security forces.
In total 471 families had fled the village to nearby villages and the provincial capital Sar-e Pul and they required urgent humanitarian assistance, the AIHRC statement said.
But the Taliban denied involvement, saying official claims implicating the group were "baseless propaganda" against it.
The AIHRC report said between 50 and 60 people, including five women and three children, had been killed in the attack, quoting a witness who said victims' hands were tied before they were shot.
UNAMA, however, urged all parties to refrain from exploiting harrowing events for political purposes and before basic facts were established.