The Bianconeri warmed up for Spurs' visit on Tuesday with a 2-0 win over Fiorentina in Serie A, a victory that moves them back on top of the table at Napoli's expense.
A couple of weeks ago Liverpool's FA Cup clash with West Brom descended into farce as the referee used VAR three times in the first half alone.
Jordan Veretout went up to take the spot-kick but after a three-minute viewing referee Marco Guida instead chose to award the visitors a free-kick for a supposed Benassi offside in the run-up.
While Jordan Veretout waited to take the penalty, the VAR (video assistant referee) was consulted.
"They took a lifetime to decide", Pioli said after the match.
"But I don't accept this decision: Alex Sandro made two touches, not just one, it's hard to believe it wasn't voluntary. And then the referee took more than three minutes to decide, how is it possible that he didn't go to look at the replay himself?"
Juventus winger Federico Bernardeschi claims he celebrated his goal against old club Fiorentina on Friday "out of respect for fans".
Friday's win also represented milestones for goalkeeper Gianluigi Buffon, who played his 500th league match for Juve, and coach Massimiliano Allegri, who managed his 200th fixture across all competitions.
"It's never easy to win here, we didn't have an awesome game, but these matches are like that, it's a big step towards the Scudetto, I'm very happy for Bernardeschi", said Argentine Higuain.
With four goals in six league starts so far this season, the Italian global is doing his best to make it hard for Juventus head coach Massimiliano Allegri to leave him out of the playing XI. "[Spurs] have excellent technique, but Champions League games are tight".