The lawsuit alleges that Prince's death was a "direct and proximate cause" of the Trinity Medical Centre's alleged failure to diagnose and treat the overdose, alongside an apparent failure to investigate the cause of it.
Prince, known for his androgynous style and sexually charged songs, died a day before he was set to meet a California-based doctor who specializes in addiction treatment.
Zimmer has accused the hospital that treated Prince and the pharmacies that filled Prince's narcotic prescriptions in the weeks before his death of deviating from standards of care.
At the time, Prince's publicist said the musician was suffering from flu-like symptoms. The suit also names Walgreens pharmacy for issuing Prince a prescription under the wrong name.
About a week earlier, his plane made an emergency stop in Moline, Illinois, when he suffered from an opioid overdose and had to be revived by paramedics.
Health officials at the hospital in IL "failed to timely and appropriately diagnose and treat opiate overdose; failed to provide timely and appropriate counseling for opiate overdose; failed to timely and appropriately investigate the cause of opiate overdose", the lawsuit said.
According to TMZ, "Prince's family claims the drug store chain was dispensing narcotic prescription medications to Prince for an invalid medical goal and failed to make sure he was using the drug properly".
Count III alleges all the defendants "failed in their duty to exercise reasonable care, failed to follow applicable IL and Minnesota law and federal regulations and/or breached the standards of care owed to Prince".
Interested in Prince? Add Prince as an interest to stay up to date on the latest Prince news, video, and analysis from ABC News. "What happened to Prince?" said Loucas. "We have client interests to protect at the moment, including our theory of the case", Zimmer said in a statement.
However, Prince's family reckon that the Trinity Medical Center and Dr Nicole Mancha should have done more following that incident. Prince's family wishes, through its investigation, to shed additional light on what happened to Prince. At the same further light on the opiate epidemic will hopefully help the fight to save lives.
Last week, no criminal charges were filed into Prince's death after a two-year inquiry.
A hospital representative told the New York Times it does not comment on pending legal matters.
Walgreens declined to comment to Reuters.