Mr Trybus faces a bail hearing on Friday on two counts of felony hate crime.
Timothy G. Trybus, the man police said harassed Irizarry, was arrested on the day of the incident on charges of assault and disorderly conduct, according to a police report.
"You should not be wearing that in the United States of America", Trybus later says in the video, moving closer to her.
When the video started gaining media attention earlier this week, politicians including Puerto Rico's governor and Cook County politicians weighed in.
"You should not be wearing that in the United States of America".
The forest preserve district says officials continue to investigate the incident.
I showed my official ID as a Member of Congress, to which the officer said, "I don't think so", indicating that she thought my ID was a fake and that I couldn't possibly be a Member of Congress.
A police officer who allegedly failed to intervene in an argument between a man and a woman over the woman's Puerto Rico flag shirt has resigned, the Chicago Sun-Times reported.
During the clip, which went on to be viewed almost two million times, Trybus yells at Irizarry that she "should not be wearing that in the United States of America" in reference to her Puerto Rican flag shirt, as well as repeatedly asking if she is an American citizen. Officer Connor was caught on camera last month, seemingly standing by as another man scolded Mia Irizarry for wearing a Puerto Rico flag T-shirt. They also said that more officers responded and arrested Trybus when the first officer asked for assistance on the radio. "Are you a United States citizen?" We can not allow those who do not understand America's greatness to terrorize people due to their background.
"Officer, I'm renting - I paid for a permit for this area".
At one point in the video, Irizarry tries to move away from Trybus and walks out from under a pavilion, but he follows her as Connor appears not to approach them.
Cook County President Toni Preckwinkle released a statement Thursday saying, "Under the leadership of General Superintendent Arnold Randall and his team, the Forest Preserves of Cook County have become a safe, welcoming place of exploration, recreation and leisure for everyone. You know that right?" says the man, who appears to be intoxicated.
Ms Irizarry used her mobile phone to film the confrontation, which unfolded on 14 June as she set up a picnic for her birthday at Caldwell Woods in the Chicago suburbs. Meanwhile an investigation into the incident continues despite the officer's resignation. The island is a USA commonwealth, and Puerto Ricans are American citizens.