They responded to their own challenge in emphatic fashion, looking more risky with the ball and sharper in the field than the world's No 2-ranked one-day side, restricting India to 256 for eight, setting themselves up for a run chase they completed at a canter to clinch a 2-1 series victory, coming from behind after losing the opening match.
This latest hundred was also Test skipper Root´s 13th in ODIs, taking him past the England record of 12 he had previously shared with Marcus Trescothick.
Under Dhoni's lead, India won the 2011 World Cup after claiming the T20 title in 2007. "We can use it as a game where it might be (like) a semi-final or a knockout game".
Kent captain Sam Billings has been called into the England one-day worldwide squad for their crucial series decider against India on Tuesday.
Dhoni too failed to get a move on in the three-match series and Ganguly said if the wicketkeeper-batsman is the team's choice for the 2019 World Cup, then he has to up his game.
David Willey and Adil Rashid finished with three wickets, whereas Mark Wood also earned a wicket.
But, after left-arm quick Willey had kept things tight early on, it was leg-spinner Rashid who did significant damage by taking two wickets in an over.
Mahendra Singh Dhoni's future has once again become fodder for speculation after the talismanic-but-currently-off-colour former India skipper sought the match ball at the end of the lost third ODI against England in Leeds. Surprised, Kohli was out for 71 off 72 balls, including eight boundaries.
Dhawan was run out for 44 by a brilliant piece of fielding from Ben Stokes and thereafter India lost wickets at regular intervals.
India could be boosted by the return of bowler Bhuvneshwar Kumar.
Kohli backed up to a delivery that turned from leg stump and knocked over his off stump. England will be looking to exploit India's middling middle-order; India will be looking to exploit England's uncertainty against Kuldeep Yadav; and both sides will be swinging for the fences.
Root clinched 100 off the last ball, hitting fast bowler Hardik Pandya through midwicket for his 10th boundary.