Williams was docked a game in the crucial second set of her match against Naomi Osaka after receiving a third code violation for calling the experienced Ramos a "thief" - and she used her subsequent post-match press conference to call her penalty "sexist".
"[It] not only exudes racist, sexist caricatures of both women, but Williams' depiction is unnecessarily sambo-like", it said in a statement. However one senior figure told the Guardian that privately there was widespread concern about how the USTA and WTA had rushed to support Williams - which had led to vitriol and abuse on social media for Ramos.
The Times of London reports that there is a growing consensus that umpires feel they were "not supported by the USTA" on several occasions and cite one anonymous source claiming that umpires are considering boycotting matches played by Williams. According to another report in The Guardian, umpires are also considering forming a union after Ramos was "hung out to dry" by the authorities despite upholding the rules. The officials are further enraged by the fact that it took International Tennis Federation (ITF) 48 hours to come to Ramos' support. Mr Ramos' decisions were in accordance with the relevant rules and were re-affirmed by the US Open's decision to fine Serena Williams for the three offences.
Ramos, 47, is the only active tennis umpire to have officiated the men's singles finals at all four grand slams. Earlier in the day, the US Tennis Association president Katrina Adams also accused Ramos, and other umpires, of gender bias in a television interview.
Williams demanded to speak to the tournament referee after the final decision, but it was not overturned.
Tennis great Billie Jean King backed the American but Margaret Court, whose major wins record Williams was trying to equal on Saturday, expressed little sympathy for the 36-year-old.
Andre Agassi got a warning for an audible obscenity at the U.S. Open and then called the umpire a "son of a bitch".
Her frustration with Ramos began when she was given a code violation for being coached in-match and breaking her racket shortly after. Osaka's victory was marred by booing from the crowd over Williams' treatment, with Williams putting her arm around a tearful Osaka and addressing the crowd.
The cartoon was intended as a lampoon of the tennis star's angry exchanges with chair umpire Carlos Ramos at the U.S. Women's Singles final in NY on Saturday. "Let's get through this". Williams then received a second violation when she smashed her racket in frustration, which caused her to lose a point.